Student Paper Award Announced!!

Session Index

Biophotonics

Oral session 1 – Biophotonics
Thursday, Dec. 4, 2014  13:30-15:00
Chair: Hiro-o Hamaguchi,Arthur Chiou
Room: B19
Notes:
13:30 - 14:00 Paper No.  2014-Thu-S0601-I003
Invited Speaker:
Hiro-o Hamaguchi
Fast, Automatic and Label-free Cell Discrimination by Raman
Hiro-o Hamaguchi,Hiro-o Hamaguchi,

Raman microspectroscopy, an alliance of Raman spectroscopy and confocal optical microscopy, is now widely used for label-free and molecular-level investigations of living cells. Here I review recent technical advances in our research group of the hardware as well as the software of Raman microspectroscopy of living cells. The advances in the hardware includes the developments of a multi-focus confocal Raman microspectrometer and a fast spiral raster scanning confocal Raman microspectrometer.These new techniques enable fast Raman imaging of living cells in several to several tens of second, an order of magnitude faster than existing commercial Raman microspectrometers. The new software utilizes the multivariate curve resolution-alternating least squares (MCR-ALS) method to obtain molecular component distribution imaging of living cells. Under appropriate model constraints, such as non-negativity and norm regularization for spectral profiles and their intensities, this new software provides physically interpretable spectral components without a priori knowledge on existing chemical components. These new software/hardware have been successfully applied to fast, automatic and label-free discrimination of living cells such as white blood cells and cardiac muscle cells.

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14:00 - 14:15 Paper No.  2014-Thu-S0601-O007
Zhen-Yi Hong Award Candidate Regeneration of zebrafish heart after site-selective injury induced with femtosecond laser ablation
Zhen-Yi Hong,Kuen-You Lin,Chung-Der Hsiao,Kazunori Okano,Ian Liau,

We developed an application of femtosecond-laser ablation to create a microscopic injury at selected sites of zebrafish heart. We analyzed the morphological and functional recovery of the zebrafish heart during regeneration. Our results might provide insight into the development of novel regenerative therapy.

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14:15 - 14:30 Paper No.  2014-Thu-S0601-O008
Yu-Yen Wang Award Candidate Full-field optical coherence tomography for threedimensional derm tissue imaging
Yu-Yen Wang,Yu-Shan Juan,Bor-Wen Yang,Bi-Xiang Cai,Jiang-Yanting Li,Shang-Ping Ying,

Full-field optical coherence tomography (FF-OCT)is suitable for rapid en-face imaging as it uses a 2D imaging device for planar interference and pixel processing of a sample plane. In this study, a red LED was applied in an FF-OCT system for skin imaging. A three-dimensional derm tissue image was obtained from the system to demonstrate the potential of LED in FF-OCT system. Noninvasive 3D skin imaging contributes to advance dermatology and cosmetology.

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14:30 - 14:45 Paper No.  2014-Thu-S0601-O017
Szu Yu Lee Award Candidate Super-resolution two-photon brain imaging with a deep penetration based on fluorescence saturation
Szu Yu Lee,Chi Kuang Sun,

Two-photon fluorescence microscopy (2PFM) features deep penetration and high resolution. However, the near infrared excitation of 2PFM results in inferior resolution to that of confocal imaging. To further enhance the resolution of 2PFM, here we propose super-resolution 2PFM. Resolution improvement is demonstrated in brain neurons imaging.

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14:45 - 15:00 Paper No.  2014-Thu-S0601-O018
Li-Wei Chu Award Candidate Combining FRET imaging with single-particle tracking elucidates autophagy mediated Dengue virus uncoating process
Li-Wei Chu,Ya-Hsuan Lin,Chau-Hwang Lee,Yueh-Hsin Ping,

We established the FRET fluorophore-labeling Dengue virus particles to visualize viral uncoating in living cells. In acceptor-bleaching imaging and single-particle tracking, the FRET signal decay was colocalized with autophagosomes. In addition, the autophagy inhibitors caused the reduction of viral uncoating process, suggesting that Dengue virus uncoating is correlated with autophagy.

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Biophotonics

Poster Session 1
Thursday, Dec. 4, 2014  15:00-16:20
Chair:
Room:
Notes:
14:40 - 15:30 Paper No.  2014-Thu-P0601-P001
Bishnubrata Patra Award Candidate Study on Vascular Formation inside a Cell Spheroid by Using Selective Plane Illumination and Confocal Microscopy
Bishnubrata Patra,Yu-Sheng Peng,Chien-Chung Peng,Wei-Hao Liao Liao,Yu-An Chen,Keng-Hui Lin,Yi-Chung Tung,Chau-Hwang Lee,

Formation of perfusable vascular networks is an important topic for tissue engineering. We cocultured human umbilical vein endothelial cells with hepatocellular carcinoma cells as spheroids of various sizes, and used selective plane illumination and confocal microscopy to monitor the structures of the endothelial cells inside a spheroid.

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14:40 - 15:30 Paper No.  2014-Thu-P0601-P006
Chun-Nien Liu Award Candidate Study of Ce-Doped Crystal Fibers Fabricated by Fiber Drawing Tower Technique
Chun-Nien Liu,Yi-Chung Huang,Pi-Ling Huang,Sheng-Lung Huang,Wood-Hi Cheng,

The fabrication of Ce-doped fibers (CeDFs) employing drawing-tower method with rod-in-tube and powder-in-tube technique is demonstrated. The fluorescence spectrum of CeDFs exhibited a 120-nm broadband emission with 1.45 μm axial resolution. This CeDF may be functioned as a high- resolution light source for optical coherence tomography (OCT) applications.

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14:40 - 15:30 Paper No.  2014-Thu-P0601-P007
Tzu-Fang Tseng Award Candidate THz near-field transmission imaging system to investigate blood absorption coefficient in-vivo
Tzu-Fang Tseng,Szu-Chi Yang,Yuan-Fu Tsai,Chi-Kuang Sun,

A THz near-field transmission type imaging system is proposed and demonstrated to investigate the blood absorption coefficient in-vivo. The in-vivo absorption coefficient of the blood of nude mouse is successfully found to be around 140cm-1 at 340GHz.

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14:40 - 15:30 Paper No.  2014-Thu-P0601-P012
Yi-Fang Wang Award Candidate The Effect of Platelets on the Viability and the Viscoelasticity of Circulating Tumor Cells under Fluid Shear Stress
Yi-Fang Wang,Yin-Quan Chen,Chien-Hsiu Huang,Zhi-hong Zhong,Muh-Hwa Yang,Arthur Chiou,

Platelets adhering to circulating tumor cells may play an import role in cancer metastasis. In this study, we reveal that platelets adhesion increases cell viscoelasticity and enhance resistance to cellular damage caused by fluid shear stress, resulting in less apoptotic cells and higher metastasis ability.

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14:40 - 15:30 Paper No.  2014-Thu-P0601-P013
Kun-Che Li Award Candidate Nonlinear Fiber Optics on Nonlinear Laser Scanning Microscopy
Kun-Che Li,Chi-Hsiang Lien,Jyan-Yo Lu,Ming-Che Chan,

Nonlinear fiber optics and nonlinear light microscopy are two totally distinct research areas. In this work, we demonstrate that by efficient wavelength-conversion mechanisms in a highly nonlinear fibers. And the fiber-delivered femtosecond 1.2-1.35 micron down-converted and 0.6-0.8 micron up-converted light sources were then utilized for nonlinear microscopy for the first time.

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14:40 - 15:30 Paper No.  2014-Thu-P0601-P018
Tze-Hung Ju Inkjet-printed cytochrome c protein for long wavelength infrared sensing
Tze-Hung Ju,Suo-Feng Liang,Guo-Dung Su,

We submitted the design of an IR microbolometer to Chip Implementation Center (CIC) in Taiwan. After we received the chips back from CIC, we ink-jetted Cytochrome c protein onto the aluminum electrode. Finally, we demonstrated the TCR of 28.27%. This value is much higher than most materials used in microbolometers.

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14:40 - 15:30 Paper No.  2014-Thu-P0601-P019
Yuan-Chin Chiu Study of Sensitivity and Resonance Response for Three-layer Structure Surface Plasmon Resonance Fiber Sensors
Hsiang-Yu Liu,Yuan-Chin Chiu,Shu-Ming Yang,Woo-Hu Tsai,

In this paper, we proposed a three-layer structure Au/ITO/Au fiber sensor based on surface plasmon resonance to improve the sensing sensitivity for the index-matching oil measurements with the refractive index ranged from 1.30 to 1.39. The results show the sensitivity can be increased 25.8% significantly.

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14:40 - 15:30 Paper No.  2014-Thu-P0601-P024
Jia-Wen Hsu Photochemical thrombosis in larval zebrafish targeting the screening of thrombolytic drugs
I-Ju Lee,Jia-Wen Hsu,Ju-Hao Tu,Ian Liau,

In light of the limitation of conventional animal models of thrombosis, we develop an application of a photochemical means to initiate thrombosis in targeted blood vessels of zebrafish larvae. This approach is extensible to induce ischemic stroke in zebrafish and may facilitate the screening of drugs with thrombolytic capability.

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14:40 - 15:30 Paper No.  2014-Thu-P0601-P025
Cheng-Hsien Lin Study of Bi-Metal Surface Plasmon Resonance Fiber Sensors with Applied Magnetic Field
Hua-Li Yeh,Cheng-Hsien Lin,Shu-Ming Yang,Woo-Hu Tsai,

We propose a new optical fiber sensor with bi-metal layers structure based on surface plasmon resonance to study the sensing sensitivity improvement under an applied external magnetic field. The results show that the magnetic field can enhance the sensing sensitivity and change the resonance absorption dip wavelength obviously.

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14:40 - 15:30 Paper No.  2014-Thu-P0601-P030
Gung-Jing He High sensitivity biosensor in a periodic array of metal nanorod pair
Gung-Jing He,Wayne Yang,Yuan-Fong Chau,

We numerically investigate a periodic array of silver-shell nanorod pair structure by using finite element method. Simulation results show that the SPR modes are very sensitive to the relative permittivities change in the surrounding materials, which could be used as highly sensitive sensors.

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14:40 - 15:30 Paper No.  2014-Thu-P0601-P031
Cheng-Han Wu Differentiate Human Leukocytes with Third Harmonic Generation and Two-photon Fluorescence Microscopy
Cheng-Han Wu,Tzu-Ming Liu,

Recognizing different types of leukocytes without a label is very helpful for flow cytometry in clinical medicine. Here we show a new method to differentiate the types of human leukocyte by using third harmonic generation and two-photon auto-fluorescence microscopy. Cell identities were confirmed by immunohistochemical fluorescence labels.

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14:40 - 15:30 Paper No.  2014-Thu-P0601-P036
Shih-Hua Chen Identified early stage mycosis fungoides from psoriasis and atopic dermatitis using non-invasive color contrast enhancement by LEDs lighting
Shih-Hua Chen,Hsiang-Chen Wang,

In this study, the spectrum of each picture element of the patient’s skin image was obtained by multi-spectral imaging technology (MSI). Spectra of normal or pathological skin were collected from 30 patients (10 early stage MF, 10 psoriasis vulgaris, and 10 atopic dermatitis). An algorithm combined with multi-spectral imaging and color reproduction technique is applied to find the best enhancement of the difference between normal and abnormal skin regions. Accordingly, an illuminant with specific intensity ratio of red, green, and blue LEDs is proposed, which has optimal color enhancement for MF detection. Compared with the fluorescent lighting commonly in the use now, the color difference between normal and inflamed skin can be improved from 11.8414 to 17.4002 with a 47% increase, 8.7671 to 12.8544 with a 26.3% increase, and 11.0735 to 17.2634 with a 34.3% increase for MF, psoriasis, and atopic dermatitis patients, respectively, thus making medical diagnosis more efficient, so helping patients receive early treatment.

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14:40 - 15:30 Paper No.  2014-Thu-P0601-P037
Zhi-Hao Jian Fiber-optic Glucose Sensor using High Sensitive Telecommunication Wavelength Modulation on Surface Plasmon Resonator
Zhi-Hao Jian,Chih-An Wei,Chi-Ting Tuan,Yu-Kun Chen,Chang-Jhe Yang,Shih-Hsiang Hsu,

The first,to our knowledge,very low glucose sensing limit(0.01mg/dL) after immobilized glucose oxidase technique was demonstrated from our surface plasmon resonator sensing with high telecommunication wavelengthresolution. Its sensitivity was showing 1.56 nm/(mg/dL).

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14:40 - 15:30 Paper No.  2014-Thu-P0601-P042
Wan-Ling Jhong Investigation of the optical property on psoralen used LED light source for PUVA
Wan-Ling Jhong,Hsi-Chao Chen,I-Lin Liu,Tzu-Hsiu Chen,Jia-Hao Zhou,

In order to whether psoralen plus UVA (PUVA) could help photochemotherapy, the transmittance and Raman spectra will be done for the detection. The results exhibited the psoralen plus UVA LED lighting source could add the transmittance and the reaction peaks in the Raman spectra.

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14:40 - 15:30 Paper No.  2014-Thu-P0601-P043
Cheng-Du Yang Surface plasmon-induced band gap in the multi-functionalized graphene oxide
Cheng-Du Yang,Nan-Fu Chiu,Chun-Chuan Kuo,

We combine surface plasmon resonance and electrochemical techniques to regulate the oxygen-containing functional groups of graphene oxide, and thus induced band gap to enhanced surface plasmon coupling effect.

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Biophotonics

Oral Session 2 - Biophotonics
Thursday, Dec. 4, 2014  15:30-17:00
Chair: Joel Yang,Shi-Wei Chu
Room: B19
Notes:
15:30 - 16:00 Paper No.  2014-Thu-S0602-I001
Invited Speaker:
Keisuke Goda
Extreme imaging and biomedical applications
Keisuke Goda,Keisuke Goda,

High-speed optical imaging is an essential tool for studying fast dynamics in plasma physics, microfluidics, phononics, and photochemistry. Unfortunately, conventional cameras based on CCD and CMOS image sensors are not fast enough to capture such events with high sensitivity. This is due to the fundamental trade-off between sensitivity and speed – at high frame rate, fewer photons are detected during each frame. In this talk, I introduce unconventional approaches to high-speed optical imaging that overcome the trade-off in the traditional image sensors and offer a record high frame rate and shutter speed without sacrificing sensitivity. I also present a new class of biomedical applications enabled by the technology such as high-throughput screening for cancer detection and drug discovery.

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16:00 - 16:30 Paper No.  2014-Thu-S0602-I006
Invited Speaker:
Joel Yang
Application of Nanoplasmonics in Color Printing
Joel Yang,Joel Yang,

The interaction of light with metal nanostructures gives rise to the collective oscillation of electrons, which can be engineered to resonate at different wavelengths by tuning the size and geometry of the structures. This phenomenon can be used to create high-resolution color prints. While the resolution of inkbased color printers is limited to ~1,000 dots-per-inch (dpi) due to the intermixing and the size of the ink drops, nanostructured metal can achieve prints at ~100,000 dpi1. At this resolution, different color pixels with dimensions of ~ 250 nm can be juxtaposed without registration errors, and color prints can be achieved at the optical diffraction limit.

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16:30 - 16:45 Paper No.  2014-Thu-S0602-O007
Chien-Chih Lan Award Candidate Cancer Cell Migration in Optical Intensity Gradients
Chien-Chih Lan,Eugene Youjhen Lu,Huei-Jyuan Pan,Chau-Hwang Lee,

We used a spatial light modulator to project optical micropatterns with an intensity gradient on lung cancer cell A549. We observed that the amounts of reactive oxygen species (ROS) in cells were in proportion to the optical intensity, and the optical intensity gradients could drive directional cell migration.

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16:45 - 17:00 Paper No.  2014-Thu-S0602-O012
Tzu-Fang Tseng Award Candidate High sensitivity of THz waves to first-stage platelet plug formation in human blood
Borwen You,Tzung-Dau Wang,Tzu-Fang Tseng,Chi-Kuang Sun,Hao-Cheng Kao,

Human blood coagulation is investigated by instant and temporal THz spectra measurement of 56 ex-vivo human blood samples either with or without adding anticoagulant. Our result indicates a high sensitivity of THz wave to the first-stage platelet aggregation and amplification process, but not the later stage blood clotting process.

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Biophotonics

Poster Session 2
Thursday, Dec. 4, 2014  16:40-17:50
Chair: Joel Yang,Shi-Wei Chu
Room:
Notes:
17:00 - 17:50 Paper No.  2014-Thu-P0602-P001
Chung-Han WANG Cytolysis of HepG2 Induced by Femtosecond Laser
Chung-Han WANG,

Process of cytolysis observed in laser treated HepG2 was analyzed by three-dimensional time-lapse imaging. Transgenic HepG2 cells expressing EGFP and YFP in cytosol and plasma membrane revealed increase of membrane permeability and generation of vesicles having plasma membrane and cytosol were generated from the laser treated cell.

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17:00 - 17:50 Paper No.  2014-Thu-P0602-P006
Jie Wang Using optical tweezers to study the effect on collagen type I viscosity caused by thermal denaturation with varying pH and additives
Jie Wang,

In this experiment, we utilized a home-made optical tweezers system to study the effect on collagen Type I viscosity caused by thermal denaturation with varying pH and additives (i.e. glycerol, urea, glucose, and NaCl). Viscosity of collagen samples were determined by measuring the thermal motion of the trapped microsphere by optical tweezers.

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17:00 - 17:50 Paper No.  2014-Thu-P0602-P007
Chiu-Jung Lai Using Spectrum Change Analysis Hydrogen Peroxide Concentration
Chiu-Jung Lai,Chia-Yu Yang,Yuan-Rong Lo,Cheng-Chih Hsu,

In this study,we will use hydrogen peroxide catalyzed by peroxidase, and then with 4-aminophenazone, phenol reacts to specific wavelengths of light product has absorption characteristics; take advantage of this feature spectral analysis of the relationship between the concentration of hydrogen peroxide.

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17:00 - 17:50 Paper No.  2014-Thu-P0602-P012
Yaping Zhang Investigation of Detection Sensitivity of Silicon Wire Biosensors
Yaping Zhang,Qinyang Sun,

Silicon wire biosensors’ detection sensitivities with TE and TM modes for typical test solutions have been investigated. It was found that the detection sensitivity of TM mode is about 7 times of that of a TE mode. Silicon wires’ detection sensitivities are a few thousand times of that of SOI rib waveguides’.

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17:00 - 17:50 Paper No.  2014-Thu-P0602-P013
Ying-Shuo Chiu Ion-sensitive field-effect-transistor-based AlGaN/GaN urea biosensors with gate recessed region using photoelectrochemical method
Ying-Shuo Chiu,Ching-Ting Lee,

The ion-sensitive field-effect-transistor-based urea biosensors were fabricated using the AlGaN/GaN heterostructure. To enhance the sensing performance, the photoelectrochemical etching and oxidation method were applied to recess the gate region and directly grow the sensing membrane, respectively. The sensing sensitivity achieved 17.5 mA/decade within the urea concentration from 0.015M to 50M.

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17:00 - 17:50 Paper No.  2014-Thu-P0602-P018
Hsiang-Wei Hsiao In-line fiber hydrogen peroxide sensor based on multimode fiber interferometer
Hsiang-Wei Hsiao,

We proposed an in-line fiber sensor to measure H2O2 concentration with multimode-fiber interferometer. The novel fiber sensor with a no-core fiber (NCF) immobilized peroxidase enzyme (POD) on surface. POD will catalyze H2O2 and lead interference wavelength shifting. The unknown H2O2 concentration sample can be obtained by the wavelength shifting signal.

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17:00 - 17:50 Paper No.  2014-Thu-P0602-P019
Hao-Cheng Gao Dispersion changes the spectral shape of third harmonic generation
Hao-Cheng Gao,Alexandra Bausch,Chien-Ming Lee,Chi-Kuang Sun,

Previous study usually believes that dependency between THG spectra and dispersion is negligible. In this letter, we show that dispersions play roles in changing the THG spectral shapes, and this effect should not be neglected for deep tissue THG spectroscopy and microscopy.

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17:00 - 17:50 Paper No.  2014-Thu-P0602-P024
Wen-Ling Lin Nanosecond-resolved Fluorescence Anisotropy Measurements on IgG Aggregation
Wen-Ling Lin,Yi-Chun Chen,

We developed a frequency-domain nanosecond-resolved fluorescence anisotropy detection system, which utilized homodyning method to record the fast dynamic fluorescence signals. We applied this technique to study immunoglobulin G (IgG) aggregation level. With our system, we were able to identify difference size of IgG aggregate from time-resolved anisotropy data.

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17:00 - 17:50 Paper No.  2014-Thu-P0602-P025
Liang-Yu Chen Comparison of CW and FD Diffuse Optical Imaging
Liang-Yu Chen,Jhao-Min Yu,Min-Chun Pan,Sheng-Yih Sun,Chia-Cheng Chou,Min-Cheng Pan,

The study aims to illustrate that both absorption and scattering optical coefficients images of breast can be reconstructed by only continuous-wave data reliably within a certain extent. Reconstructed images for phantoms with designated absorption / scattering properties of inclusion and background are presented to demonstrate this perspective.

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17:00 - 17:50 Paper No.  2014-Thu-P0602-P028
Jia Shiuan Wu Electric field enhancements around a pair of nanorod on a substrate
Jia Shiuan Wu,Yuh-Sien Sun,Gung-Jing He,Yuan-Fong Chau,

Electric field distributions of a pair of silver nanorod on a substrate are performed by the finite element method. We find the incident waves are reflected back by the substrate and the superposition of the electric fields of the incident wave and the reflection wave.

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17:00 - 17:50 Paper No.  2014-Thu-P0602-P029
Chun-Hui Yu A Fully Digital Stimulated Emission Microscopy System
Shen ShouMax Chung,Chun-Hui Yu,Fu-Jen Kao,

Stimulated Emission (SE) has distinctive characteristics different from fluorescence lifetime image microscopy (FLIM) in that the coherence nature of SE signals enables long distance observation. In SE microscopy, the coincidences of pump and probe beams in space and time are essential. Previously the coincidence in time was accomplished by translational stage and delay cables, now we can accomplish this by a fully digital system, in which the relative delay between pump and probe beam is controlled by a function generator and delay generator, and delay can be adjusted as short as 30ps.

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Biophotonics

Oral Session 4 - Biophotonics
Friday, Dec. 5, 2014  09:30-11:00
Chair:
Room: B19
Notes:
09:30 - 09:45 Paper No.  2014-FRI-S0603-O002
Yong Da Sie Fabrication of Multi-Layer 3D Biopolymer Microstructures via Multiphoton Excited Photochemistry
Yong Da Sie,Nan-Shan Chang,Shean-Jen Chen,

To fabricate complex 3D structure with nature proteins and overcome the nature limitation of fibronectin (FN) stacking in 3D conditions, bovine serum albumin (BSA) protein can easily form complex 3D scaffold via multiphoton excited photochemistry, then FN can be fabricated at specific location on BSA scaffold to help and guide cell growing three-dimensionally

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09:45 - 10:00 Paper No.  2014-FRI-S0603-O005
Jia-Wen Hsu Zebrafish models of ischemic and hemorrhagic stroke induced with photochemical thrombosis and ablation
Jia-Wen Hsu,I-Ju Lee,Yen-Ling Chen,Ian Liau,

We developed two laser based methods – photochemical thrombosis and femtosecond laser ablation – to induce ischemic and hemorrhagic stroke at selected cerebral blood vessels of zebrafish larvae. Our novel zebrafish models of stroke combined with fluorescent imaging facilitate visualization of neovascularization and rerouting of cerebral vessels after stroke.

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10:00 - 10:15 Paper No.  2014-FRI-S0603-O008
Lan-lin Jang Optical Measurement on Cell Membrane Roughness Influenced by Paclitaxel and Gold Nanoparticles
Lan-lin Jang,

We measured the membrane roughness of neuroblastoma cells under the treatments of gold nanoparticles (AuNPs) and paclitaxel. The positive charges on AuNPs reduced the roughness of cell membranes. The stabilization of intracellular microtubules by paclitaxel led to similar results.

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10:15 - 10:30 Paper No.  2014-FRI-S0603-O011
Zhung-Fu Lee Using optical coherence tomography to investigate temporal effects induced by microneedle for transdermal drug delivery
Zhung-Fu Lee,Ting-Yen Tsai,Feng-Yu Chang,I-Chi Lee,Chih-Hsun Yang,Meng-Tsan Tsai,

Transdermal drug delivery systems (TDDS) have been an attracting field in drug delivery because of its benefit over parenteral and oral administration. Recent studies illustrate that microneedle (MN) can penetrate through stratum corneum (SC) barrier without damaging nerves. In this study, we used optical coherence tomography (OCT) to investigate the temporal effects on skin induced by MN for transdermal drug delivery. With OCT, the morphological changes in skin due to MN can be identified from 2D or 3D OCT image. Moreover, the penetration depth and damaged area can be quantitatively evaluated. In addition, OCT was used to reconstruct micro-angiography of mouse skin and to observe the changes in micro-angiography induced by MN. Finally, OCT is implemented for dynamic evaluation of skin recovery after using MN array for drug delivery.

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10:30 - 10:45 Paper No.  2014-FRI-S0603-O014
Szu Yu Lee Submicron Photoacoustic Microscopy with High Imaging Rate
Szu Yu Lee,Chi Kuang Sun,

Conventional Photoacoustic microscopy (PAM) enhances ultrasonically determined resolution at the expense of penetration depth. Also, high resolution PAM usually uses time consuming mechanical scanning, which lacks of practicality. In this paper, we propose a high frame rate laser scanning PAM, which features an optically determined submicron resolution.

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10:45 - 11:00 Paper No.  2014-FRI-S0603-O017
Ryosuke Oketani Modification of Point-Spread Function in Confocal Imaging by Using Nonlinear Light Scattering
Ryosuke Oketani,Hsueh-Yu Wu,Hsuan Lee,Yen-Ta Huang,Yasuo Yonemaru,Tung-Yu Su,Satoshi Kawata,Shi-Wei Chu,Katsumasa Fujita,

We experimentally observed the change of imaging property of confocal microscopy during observation of a gold nanoparticle with different illumination intensities. Modification of the point-spread function through the nonlinear nature of plasmonic scattering, which has been reported previously, was confirmed. We extracted the nonlinear scattering components for high spatial-resolution imaging.

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Biophotonics

Oral Session 5 - Biophotonics
Friday, Dec. 5, 2014  11:10-12:10
Chair:
Room: B19
Notes:
11:10 - 11:25 Paper No.  2014-FRI-S0604-O002
Chao-You Hung Write-Read-Erase DNA Biopolymer Memory Devices
Yi-Tzu Lin,Waan-Ting Tu,Chao-You Hung,Yu-Chueh Hung,

Deoxyribonucleic acid (DNA) biopolymer material has been attracting great interest for years and became a promising material for many optoelectronic applications. In this study, we reported the fabrication of the DNA biopolymer with silver nanoparticles using a photochemical method and demonstrated a device performing a write-read-erase memory characteristic.

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11:25 - 11:40 Paper No.  2014-FRI-S0604-O003
Yi-Chang Wu Intracellular Stiffness of Different Epithelial-Mesenchymal Transition Phenotypes Studied by Video Particle-Tracking Microrheology
Yi-Chang Wu,Arthur Chiou,

Epithelial–mesenchymal transition (EMT), a crucial process in tumor metastasis, is often associated with the reorganization and restructuring of actin cytoskeletons, resulting in a change in intracellular stiffness. We measured the intracellular stiffness at different stages of EMT for potential application in the evaluation of metastatic potential of cancer cells.

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11:40 - 11:55 Paper No.  2014-FRI-S0604-O008
Cheng Nai-Lun Portable LED Induced Autofluorescence (LIAF) imager for Oral Cancer Diagnosis by RGB ratio
Cheng Nai-Lun,Tsai Ming-Hsui,Chiou Jin-Chern,Duann Jeng-Ren,Lin Yung-Jiun,Huang Ting-Wei,Yang Chin-Siang,Li Sing-Tsung,Liu Ying,Ou-Yang Mang,Hsieh Yao-Fang,

We developed a portable LED induced autofluorescence (LIAF) imager through 365nm and 472nm wavelength as excitation light sources to capture ex-vivo oral tissue autofluorescence image. The device achieves a sensitivity of 87.26% and specificity of 62.90% when using normalized blue-to-green ratio analysis method on autofluorescence image with 365nm excitation light source.

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11:55 - 12:10 Paper No.  2014-FRI-S0604-O011
Ting-Yen Tsai Evaluation of fractional laser-assisted drug delivery with optical coherence tomography
Ting-Yen Tsai,Chih-Hsun Yang,Su-Chin Shen,Chau Yee Ng,Shih-Ming Jung,Meng-Tsan Tsai,

To ensure proper energy delivery without damaging tissue structures beneath the nail plate, optical coherence tomography (OCT) was implemented for quantitative evaluation of induced microscopic ablation zone (MAZ). To further study the feasibility of drug delivery, normal saline was dripped on the exposure area of fingernail and the speckle variance in OCT signal was used to observe water diffusion through the ablative channels into the nail plate.

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Biophotonics

Oral Session 3 - Biophotonics
Friday, Dec. 5, 2014  15:40-17:10
Chair: Ja-Yu Lu,Yi-Chun Chen
Room: B19
Notes:
08:00 - 08:15 Paper No.  2014-FRI-S0606-O002
Kazunori Okano Ablation of targeted single cardiomyocyte of larval zebrafish in vivo with a femtosecond laser
Kazunori Okano,Kuen-You Lin,Chung-Han Wang,Ian Liau,

The heart of larval-zebrafish was ablated with a femtosecond laser in vivo under asystole. Fluorescence image of transgenic fish expressing EGFP-myosin and mCherry-histone revealed that a targeted cardiomyocyte was ablated. Vesicles that contained cytosol and were enclosed by plasma membrane were produced immediately after ablation, which was followed by cytolysis.

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08:15 - 08:30 Paper No.  2014-FRI-S0606-O006
Sandeep Chakraborty Fluorescence Lifetime Imaging of Endogenous Fluorophores Reveals Differences between Normal and Parkinson’s Disease Induced Cells
Sandeep Chakraborty,Chiao-Ming Huang,Artashes Karmenyan,Jin-Wu Tsai,Arthur Chiou,

We applied two photon fluorescence lifetime imaging microscopy (TP-FLIM) to determine the fluorescence lifetime as well as the amounts of endogenous fluorophores, NADH and FAD, in normal and Parkinson’s disease induced PC12 cells. Our results show a significant difference in cellular metabolic state in normal vs. diseased cells.

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08:30 - 08:45 Paper No.  2014-FRI-S0606-O009
Yen-Ling Chen Pseudodynamic 3D cardiac imaging reveals distinct functional aspects of larval zebrafish heart
Yen-Ling Chen,Zhen-Yi Lin,Zhen-Yi Hong,Ian Liau,

We apply pseudodynamic 3D imaging to assess the two-chambered heart of zebrafish larvae. We reveal distinct functional aspects of the heart of larval zebrafish with emphasis on the subtle difference relative to the heart tube of zebrafish embryos and the four-chambered human heart.

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08:45 - 09:00 Paper No.  2014-FRI-S0606-O016
Chi-Hsiang Lien Temporal focusing multiphoton microscopy with astigmatism imaging method
Chi-Hsiang Lien,Chun-Yu Lin,Shean-Jen Chen,Fan-Ching Chien,

A three-dimensional (3D) single fluorescent particle tracking strategy based on temporal focusing multiphoton excitation microscopy (TFMPEM) combined with astigmatism imaging is proposed for delivering nanoscale-level axial information that reveals 3D trajectories of single fluorospheres in the axially-resolved multiphoton excitation volume without z-axis scanning.

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09:00 - 09:15 Paper No.  2014-FRI-S0606-O019
Ting Huo Polycrystalline Silicon Nanowire Ammonia Sensors
Ting Huo,

Ammonia sensors are easily affected by relative humidity (RH) when performing electrical measurement. In this paper, we have found that this undesirable effect can be improved by applying cyclohexane-triphenylene (cchxnTP) as modification layers of nanowire devices. We also discuss the relation between surface modification and morphology in sensing mechanisms.

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09:15 - 09:30 Paper No.  2014-FRI-S0606-O025
Chin Hao Chang In vivo Eyes Development of Drosophila in Embryonic Stage Studied by Three-dimensional Time-lapsed Imaging via Multi-channel Single Plane Illumination Microscopy (SPIM)
Chin Hao Chang,Yu Shan Huang,Yun Chi Tsai,Hui Yu Ku,Arthur Chiou,Y. Henry Sun,

A multi-channel Single Plane Illumination Microscopy (SPIM) has been established and applied to study the cell dynamics during the Eye-Antenna Disc Primordium (EADP) formation of Drosophila melanogaster. The contribution of en gene to eyes development in embryonic stage has also been verified by comparing the dual-channel in vivo three-dimensional time-lapsed images of the expression patterns of CD enhancer and en gene.

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Biophotonics

Oral Session 6 - Biophotonics
Friday, Dec. 5, 2014  15:40-17:10
Chair:
Room: B19
Notes:
15:40 - 15:55 Paper No.  2014-FRI-S0605-O001
Liang-Yu Chen Multi-wavelength Diffuse Optical Imaging
Liang-Yu Chen,Min-Chun Pan,Min-Cheng Pan,

Diffuse optical imaging can show the map of chromophore concentration in the medium from multi-wavelength light measurement. In this study, initial simulation of diffuse optical imaging for reconstructing chromophore concentration, such as oxy-hemoglobin, deoxy-hemoglobin, and water, is presented.

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15:55 - 16:10 Paper No.  2014-FRI-S0605-O002
Hung Van Nguyen Measurement of Refractive index of liquids using low-coherence fiber-optic Fabry-Perot interferometer
HungVan Nguyen,Shyh-Tsong Lin,

In this article, the mathematical analysis and experiment set up of low-coherence fiber optic Fabry-Perot interference have been demonstrated. Since we want to confirm the ability in determining refractive index of liquid, a series of test with water samples and without sample were conducted under favorable conditions.

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16:10 - 16:25 Paper No.  2014-FRI-S0605-O008
Yu-Han Chang Non-invasive method to evaluate hepatic functions by two-photon fluorescence microscopy of intravenous indocyanine green (ICG)
Yu-Han Chang,Kai-Wen Huang,Yao-Ming Wu,Tzu-Ming Liu,

We developed an in vivo non-invasive detection system with two-photon fluorescence to conduct a preoperative assessment of hepatic functions. In our rat models, the residual ICG on 15 minutes post administration (ICG-R15) in rats with hepatocellular carcinoma is above 40%; control group, below 15%. It's as accurate as traditional methods.

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16:25 - 16:40 Paper No.  2014-FRI-S0605-O009
Chia-Ta Tseng Simulation analysis of the angular span of the Optical Phase Conjugation Phenomenon using pseudospectral time-domain technique
Chia-Ta Tseng,Snow H. Tseng,

The pseudospectral time-domain (PSTD) technique is employed to obtain numerical solutions of Maxwell’s equations. Outgoing light is recorded and used to generate phase-conjugated light which back-propagates through the scattering medium. By changing the angular span, we can analyze different angular spans and help determine the optimal angular span for back-propagation of light.

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16:40 - 16:55 Paper No.  2014-FRI-S0605-O016
Shi-Hao Huang OCT tomographic reconstruction of melanin structure via finite-difference time-domain simulation
Shi-Hao Huang,Shiang-Jiu Wang,snow H Tseng,

The finite-difference time-domain method (FDTD) can help us to analyze data acquired in the optical coherence tomography (OCT). We reconstruct the structure of melanin, which is the key component of human pigment system. Simulation reconstruction shows structures of the melanin that may be useful for biomedical optics application.

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16:55 - 17:10 Paper No.  2014-FRI-S0605-O017
Chia-Wei Lee Nonlinear structured-illumination enhanced temporal focusing multiphoton excitation microscopy with a digital micromirror device
Li-Chung Cheng,Chia-Wei Lee,Chi-Hsiang Lien,Yong Da Sie,YulingYvonne Hu,Chun-Yu Lin,Fan-Ching Chien,Chris Xu,Chen Yuan Dong,Shean-Jen Chen,

The light diffraction of temporal focusing multiphoton excitation microscopy (TFMPEM) and the excitation patterning of nonlinear structured-illumination microscopy (NSIM) can be simultaneously implemented via a single high-resolution digital micromirror device. The lateral and axial spatial resolutions of the TFMPEM are remarkably improved through the second-order NSIM and structured light, respectively.

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