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08:00 - 08:15
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Paper No. 2014-FRI-S0606-O004
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| Kazunori Okano
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Ablation of targeted single cardiomyocyte of larval zebrafish in vivo with a femtosecond laser
Kazunori Okano,Kuen-You Lin,Chung-Han Wang,Ian Liau,
The heart of larval-zebrafish was ablated with a femtosecond laser in vivo under asystole. Fluorescence image of transgenic fish expressing EGFP-myosin and mCherry-histone revealed that a targeted cardiomyocyte was ablated. Vesicles that contained cytosol and were enclosed by plasma membrane were produced immediately after ablation, which was followed by cytolysis.
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08:15 - 08:30
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Paper No. 2014-FRI-S0606-O008
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| Sandeep Chakraborty
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Fluorescence Lifetime Imaging of Endogenous Fluorophores Reveals Differences between Normal and Parkinson’s Disease Induced Cells
Sandeep Chakraborty,Chiao-Ming Huang,Artashes Karmenyan,Jin-Wu Tsai,Arthur Chiou,
We applied two photon fluorescence lifetime imaging microscopy (TP-FLIM) to determine the fluorescence lifetime as well as the amounts of endogenous fluorophores, NADH and FAD, in normal and Parkinson’s disease induced PC12 cells. Our results show a significant difference in cellular metabolic state in normal vs. diseased cells.
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08:30 - 08:45
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Paper No. 2014-FRI-S0606-O017
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| Yen-Ling Chen
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Pseudodynamic 3D cardiac imaging reveals distinct functional aspects of larval zebrafish heart
Yen-Ling Chen,Zhen-Yi Lin,Zhen-Yi Hong,Ian Liau,
We apply pseudodynamic 3D imaging to assess the two-chambered heart of zebrafish larvae. We reveal distinct functional aspects of the heart of larval zebrafish with emphasis on the subtle difference relative to the heart tube of zebrafish embryos and the four-chambered human heart.
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08:45 - 09:00
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Paper No. 2014-FRI-S0606-O024
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| Chi-Hsiang Lien
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Temporal focusing multiphoton microscopy with astigmatism imaging method
Chi-Hsiang Lien,Chun-Yu Lin,Shean-Jen Chen,Fan-Ching Chien,
A three-dimensional (3D) single fluorescent particle tracking strategy based on temporal focusing multiphoton excitation microscopy (TFMPEM) combined with astigmatism imaging is proposed for delivering nanoscale-level axial information that reveals 3D trajectories of single fluorospheres in the axially-resolved multiphoton excitation volume without z-axis scanning.
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09:00 - 09:15
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Paper No. 2014-FRI-S0606-O032
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| Ting Huo
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Polycrystalline Silicon Nanowire Ammonia Sensors
Ting Huo,
Ammonia sensors are easily affected by relative humidity (RH) when performing electrical measurement. In this paper, we have found that this undesirable effect can be improved by applying cyclohexane-triphenylene (cchxnTP) as modification layers of nanowire devices. We also discuss the relation between surface modification and morphology in sensing mechanisms.
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09:15 - 09:30
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Paper No. 2014-FRI-S0606-O038
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| Chin Hao Chang
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In vivo Eyes Development of Drosophila in Embryonic Stage Studied by Three-dimensional Time-lapsed Imaging via Multi-channel Single Plane Illumination Microscopy (SPIM)
Chin Hao Chang,Yu Shan Huang,Yun Chi Tsai,Hui Yu Ku,Arthur Chiou,Y. Henry Sun,
A multi-channel Single Plane Illumination Microscopy (SPIM) has been established and applied to study the cell dynamics during the Eye-Antenna Disc Primordium (EADP) formation of Drosophila melanogaster. The contribution of en gene to eyes development in embryonic stage has also been verified by comparing the dual-channel in vivo three-dimensional time-lapsed images of the expression patterns of CD enhancer and en gene.
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