Process of cytolysis observed in laser treated HepG2 was analyzed by three-dimensional time-lapse imaging. Transgenic HepG2 cells expressing EGFP and YFP in cytosol and plasma membrane revealed increase of membrane permeability and generation of vesicles having plasma membrane and cytosol were generated from the laser treated cell.

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In this experiment, we utilized a home-made optical tweezers system to study the effect on collagen Type I viscosity caused by thermal denaturation with varying pH and additives (i.e. glycerol, urea, glucose, and NaCl). Viscosity of collagen samples were determined by measuring the thermal motion of the trapped microsphere by optical tweezers.

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In this study,we will use hydrogen peroxide catalyzed by peroxidase, and then with 4-aminophenazone, phenol reacts to specific wavelengths of light product has absorption characteristics; take advantage of this feature spectral analysis of the relationship between the concentration of hydrogen peroxide.

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Silicon wire biosensors’ detection sensitivities with TE and TM modes for typical test solutions have been investigated. It was found that the detection sensitivity of TM mode is about 7 times of that of a TE mode. Silicon wires’ detection sensitivities are a few thousand times of that of SOI rib waveguides’.

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The ion-sensitive field-effect-transistor-based urea biosensors were fabricated using the AlGaN/GaN heterostructure. To enhance the sensing performance, the photoelectrochemical etching and oxidation method were applied to recess the gate region and directly grow the sensing membrane, respectively. The sensing sensitivity achieved 17.5 mA/decade within the urea concentration from 0.015M to 50M.

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We proposed an in-line fiber sensor to measure H2O2 concentration with multimode-fiber interferometer. The novel fiber sensor with a no-core fiber (NCF) immobilized peroxidase enzyme (POD) on surface. POD will catalyze H2O2 and lead interference wavelength shifting. The unknown H2O2 concentration sample can be obtained by the wavelength shifting signal.

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Previous study usually believes that dependency between THG spectra and dispersion is negligible. In this letter, we show that dispersions play roles in changing the THG spectral shapes, and this effect should not be neglected for deep tissue THG spectroscopy and microscopy.

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We developed a frequency-domain nanosecond-resolved fluorescence anisotropy detection system, which utilized homodyning method to record the fast dynamic fluorescence signals. We applied this technique to study immunoglobulin G (IgG) aggregation level. With our system, we were able to identify difference size of IgG aggregate from time-resolved anisotropy data.

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The study aims to illustrate that both absorption and scattering optical coefficients images of breast can be reconstructed by only continuous-wave data reliably within a certain extent. Reconstructed images for phantoms with designated absorption / scattering properties of inclusion and background are presented to demonstrate this perspective.

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Electric field distributions of a pair of silver nanorod on a substrate are performed by the finite element method. We find the incident waves are reflected back by the substrate and the superposition of the electric fields of the incident wave and the reflection wave.

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Stimulated Emission (SE) has distinctive characteristics different from fluorescence lifetime image microscopy (FLIM) in that the coherence nature of SE signals enables long distance observation. In SE microscopy, the coincidences of pump and probe beams in space and time are essential. Previously the coincidence in time was accomplished by translational stage and delay cables, now we can accomplish this by a fully digital system, in which the relative delay between pump and probe beam is controlled by a function generator and delay generator, and delay can be adjusted as short as 30ps.

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